![]() ![]() However, parasitological methods and coprological analysis (especially eggs) in feces remain the gold standard for this detection. The advantages of this method can help in the identification and molecular characterization of parasite species. This is the first time that such a technique has been used to detect these nematodes in fecal samples. describe a novel PCR-based method for the diagnosis of Enterobius vermicularis in stool samples, specifically designed for clinical application. used a similar technique to accurately identify and detect Staphylococcus aureus in clinical samples. hydrophila infection and on-site detection, especially in resource-poor areas of this pathogen in food and aquaculture. The authors showed the prospect of this promising method in the early diagnosis of A. The results of the expression of the genes can be read using a UV flashlight. This method is rapid and highly sensitive. hydrophila is a re-emerging aquaculture and waterborne/foodborne pathogen to humans. reported the development of the visual method, dRAA-CRISPR/Cas12a, in detecting the expression of the virulent genes aerA and/or hlyA of Aeromonas hydrophila. This Research Topic includes but is not limited to (i) progression of methodology for pathogen diagnostics, (ii) sequencing-based applications in diagnostics of clinical infection, (iii) progression of databases and analysis procedures for clinical application of metagenomics, (iv) CRISPR-Cas based pathogen identification, and (v) discovery of candidate biomarkers for infection diagnostics. This Research Topic evaluates these new possibilities and brings together authorities in this field and experiences that will focus on the clinical applications, advantages, and challenges of all aspects of molecular approaches for pathogen diagnostics. In light of these challenges, new research strategies have appeared that enhance the possibility of traditional pathogen detection methods. ![]() Although mNGS and SHERLOCK have played an active role in diagnosing clinical infection, they still face many limitations and challenges in clinical applications (e.g., quality control and standardization, distinguishing pathogenic pathogens from colonized pathogens, stability, simplicity, and diversity of targets). It has been used to detect viruses such as Zika, dengue, and African classical swine fever virus. Specific High Sensitivity Enzymatic Reporter UnLOCKing (SHERLOCK) is a platform molecular diagnostic based on CRISPR-Cas enzymology for specific recognition of desired DNA or RNA sequences. It has been recognized as a subversive technique for pathogen detection, especially for rare and complex infectious diseases. Metagenomic next-generation sequencing (mNGS) is a powerful pathogen detection approach that can potentially diagnose nucleic acids (DNA and RNA) of all infectious diseases, including bacteria, fungi, viruses, and parasites, in a single test. Therefore, there is an urgent need for a series of more powerful pathogen detection tools. Traditional detection methods cannot effectively address them. However, these methods have many limitations, such as long detection cycles, low sensitivity, and narrow detection spectrum of pathogens, especially for infections caused by rare or new pathogens. The traditional etiological diagnosis methods of infection mainly include morphological identification, microbial culture, smear microscopic examination, antigen and antibody detection, nucleic acid detection, etc. Therefore, etiological detection technology is required to achieve timely, accurate, and comprehensive detection of pathogens. ![]() The diagnosis and treatment depend on etiological detection techniques. The incidence of infectious diseases is high and often causes critical illness, which seriously threatens human life and health. In this Research Topic of Frontiers in Microbiology, we assembled a collection of 12 original research articles and 2 reviews within the theme “ Molecular Diagnostics for Infectious Diseases: Novel approaches, Clinical Applications and Future Challenges.” The intended goal of this Research Topic was to present an updated view of current innovations in molecular diagnostics and challenges against clinical pathogens.
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